Search results for " separation"
Article
Alternative solutions to separate AAV full and empty capsids using anion exchange
Here we present high-performance anion exchange (AEX) chromatography separation for several AAV serotypes using step-elution mode. Optimized protocols and conditions for both resin and membrane format…
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How to optimize AAV capsid separation
In this free, interactive course you’ll learn how to tackle one of the key challenges in Adeno-associated virus (AAV) manufacturing–separating full and empty capsids in the polishing step. You’ll …
Resource
CASSS — An International Separation Science Society
CASSS is a not-for-profit 501(c)(6) professional society made up of over 4,000 industry, academic and public sector professionals in the field of separation science and related fields.
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Tools and Solutions for Separation of Charged mAb Variants
A biosimilar is an almost identical version of an originator product. To attain regulatory approval, a comparable quality to the reference product in terms of efficacy, purity, and safety should be …
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SEC in the Modern Downstream Purification Process
…ng purified for further processing or formulation and storage without having an effect on the actual separation. This lack of chemical interaction allows SEC to have a significant advantage over othe…
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Process Chromatography: Continuous Optimization
Drug manufacturers are always seeking ways to improve the efficiency and productivity of chromatographic separations. Suppliers of chromatography resins are developing new resin chemistries and bindin…
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N-Glycan Analysis of Biotherapeutic Proteins
N-glycan labeling
Released N-glycans are frequently labeled with a fluorophore to enhance glycan separation and detection (34). Commonly used labels include 2-aminobenzamide (2-AB) and 2-aminob…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
…eity of proteins, yet despite good resolving power and robustness, ionic strength-based ion exchange separations are product-specific and time-consuming to develop. Some improvements have been made t…
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Process Chromatography Selection for Downstream Processing Applications
Selecting the appropriate separation technology plays a big role in maximizing productivity in any downstream processing application. Some factors companies may consider when selecting a separation te…
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A Look at the Affinity Chromatography Landscape
Specific binding interactions between biologic molecules and immobilized affinity ligands allow for selective separations. With the advent of next-generation biopharmaceuticals, chromatography materia…